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The equipment for a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was arranged in a proper way as well as for every gel. There was the distance of 1mm spacers between the two lead crystal plates. Then, the plastic comb was put in and it was noted that the comb penetration must be noticed. In the next step, detached the comb and other two similar SDS-PAGE determining gels (Table) were equipped and dispensed in in the middle of the glass plates to almost 0.5-1 cm under the lowest of the comb.

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The four stages of sexual response according to Master and Johnson (1966) are considered the steps to a happy correlation between the men and the women. One cannot be without the other one, the four phases the both male and female experience in a sexual response cycle are excitement, plateau, orgasm, and resolution. Sexual response cycle refers to the series of physical and emotional changes that occur between two persons as they become sexually aroused and participate in sexually stimulating activities. By knowing how you respond to these cycles your relationship can be improve and help you identify any sexual dysfunction.

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Sexual dysfunction according to the Taber’s Cyclopedic Medical Dictionary is inadequate enjoyment of sex, or complete failure to enjoy any form of sexual intercourse. There may be multiple causes for the lack of sexual interest or desire, impairments in sexual arousal such as erectile dysfunction in men or premature ejaculation or vaginismus or hypoactive sexual desire disorder (HSDD) in woman. Many cases may be attribute to substance abuse, alcoholism, prescription drugs, hormonal conditions in woman, or may be a case of rape or sexual abuse during childhood. However, other reasons for both men and women having a sexual dysfunction are related to psychological reasons than to sexual dissatisfaction.

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The United States is not like any other country in the world; the freedoms experience here and education surpass any other country. People come here to the United States looking to live the American dream but sometimes become a nightmare. For many people to express themselves when they do not speak the language is very difficult. The language barrier is one of the causes, the English language is difficult for some people to learn and others don’t make the effort to learn.

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Famous poets wrote and quote “Give me your tired, your poor, your huddle masses yearning to breathe free, the wretched refuse of your teeming shore. Send these, the homeless, tempest-tots’ to me; I lift my lamp beside the golden door!”(Emma Lazarus, 1883). The United States is considered the land of freedom were for hundred of year people has come looking to lived the American dream. They come for the freedom, jobs, and in many cases to practice their religion without been persecuted. But the immigration issue has cost this nation a lot to be concern and now the fragile balance to support immigration to this country has been compromised.

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